Effect of ph on catalase activity the pingry school. This homogenate was then filtered through threelayered cheese close and 25 ml of the filtrate was obtained. Without her, we wouldnt have been able to create this guide. For the reaction we are studying in this weeks lab. The purpose of this experiment is to learn the effects of a certain enzyme peroxidase concentration, to figure out the temperature and ph effects on peroxidase activity and the effect of an inhibitor. Use forceps to push the 100 oc disc to the bottom of the room temperature hydrogen peroxide. In this investigation, will focus on the enzyme catalase in the reaction. If we titrate a solution of h 2 o 2 uncovered for 24 hours, distilled water, and h 2 so 4, then we could determine the uncatalyzed rate of decomposition of h 2 o 2. In this lab activity, you will study the enzyme catalase. The purpose of this lab is to determine if changes in temperature will have an effect on the activity of an enzyme.
Catalase accelerates the breakdown of hydrogen peroxide into water and oxygen. All raw data is stated in graphs at the end of this report. When hydrogen peroxide h 2 o 2 is added to liver, a chemical reaction occurs which results in the products of oxygen gas o 2 and liquid water h 2 o. Annie chiang ib biology hl period 2 catalase activity lab introduction. To examine the rates of enzymatic reactions and effects of varying applied conditions. You need to use these guidelines to write the report. You will not be allowed in the lab without these minimum requirements. The independent variables were time and the ratio of enzyme to substrate concentration and the dependent variable was the amount of reaction that occurred. The purpose of a biology lab report is to describe and analyze a laboratory experiment that explores a scientific concept so that other scientists might be able to replicate the experiment and investigate the principles of it. In this experiment, using agarose gel electrophoresis, the number and relative positions of restriction sites for three restriction enzymes, ecor1, hincii and pvuii, on the circular plasmid pbr322 were mapped by determining the length in base pairs of the dna fragments obtained when cutting the plasmid with each of the restriction enzymes separately and each combination thereof. An enzyme is a biological catalyst that increases the rate of chemical reaction by.
Pdf this report examines the effect of changing substarte concentration on. Write a balanced chemical equation with state symbols for the reaction catalyzed by peroxidase. Time how long it takes the disc to rise to the top. As could be predicted, the purified enzyme from a common soil fungus has a ph optimum of 5. If hydrogen peroxide is a liquid, what might you expect to see when it decomposes. Enzyme lab report bio181l general biology i lab gcu.
Substrate concentration, temperature, and ph all affect the chemical reaction. In this lab, enzyme kinetics are examined utilizing various experimental techniques, including measurements of absorbance and temperature, to determine the effects on reaction rate dependent on enzyme and. The enzyme concentration showed a steady decrease in the rate of reaction in relation to increased concentration. The assay system used in this lab consists of a filter paper disc coated with the enzyme and then pushed to the bottom of a beaker of substrate hydrogen peroxide. If the reaction is complete, then the hydrogen peroxide. In this lab, you will study an enzyme that is found in the cells of many living tissues. Investigating enzymes effect of temperature on enzyme. Enzyme lab effect of temperature and ph on amylases ability to transform starch into maltose sugar amylase amylase is an enzyme that isfound in our bodies that functions to help the body in the digestion food.
As the catalase breaks down the hydrogen peroxide into water and. Catalase enzymes abstract in this laboratory exercise, studies of enzyme catalase, which accelerates the breakdown of. The reaction studied was the breakdown of hydrogen peroxide catalyzed by the enzyme peroxidase. Katie kinoshita lab report 042718 enzymes lab purpose. Using quantitative as well as qualitative observation the concept that enzymes. Catalase is an enzyme that breaks down hydrogen peroxide into water and gaseous oxygen. An investigation of catalasehydrogen peroxide enzymatic.
The enzyme peroxidase case study lab report autumn vick i. Effect of ph on enzyme activity each enzyme has a ph at which the rate of the reaction is optimal. This lab was run to determine whether ph would affect the rate of an enzyme catalyzed reaction. They visited a writing center professional development meeting and graciously talked to us. The purpose of a lab report is to communicate the findings of your scientific study. Determining the catalytic properties of the enzyme peroxidase extracted from a turnip under the conditions of temperature, ph, boiling and competitive inhibitors by robin caserta bio 101 september 30, 20 abstract the enzyme, peroxidase, extracted from a turnip was tested for its efficiency in binding to its substrate and its stability under several conditions. The reaction in question was the breakdown of hydrogen peroxide catalyzed by the enzyme peroxidase into oxygen and water. All organisms rely on enzymes to catalyze chemical reactions. Objectives after completing this exercise you should be able to. The reaction that will be investigated in this experiment is the decomposition of hydrogen peroxide.
Pdf che506 lab report on investigation on enzymes and. Read this science lab report and over 89,000 other research documents. The substrate, the substance that is acted upon by the enzyme, is hydrogen peroxide h 2 o 2. The purpose of this lab was to help students understand how heat can make a difference on enzymes. Enzymes are proteins that catalyze chemical reactions increase the rates of biological reactions. Take a look at this lab report for a better understanding of how enzymes work. The amount of h 2 o 2 decomposed is equivalent to the baseline determined in activity b minus the change in volume from table 2. The enzyme sucrase will only act on the sugar called sucrose because it is the only substrate that can fit into the active site of the sucrase enzyme.
The purpose of this lab was to test enzyme function in various environments. An enzyme is a protein and is a catalyst to chemical reactions. Without enzymes, the process of metabolism would be hopelessly slow. When hydrogen peroxide h2o2 was added to the liver in test tube 2, the catalase in the river bonded with the peroxide the substrate, forming the catalaseperoxide complex. It helps accelerate reactions by lowering the activation energy, which is needed for reactions in cells to progress at a higher rate. It was confirmed in this experiment that changes in the environment like temperature, ph levels, substrate and enzyme concentrations did effect the rate of. This is an equilibrium reaction, and will be favored by a high concentration of enzyme andor substrate. The enzyme remains unchanged throughout the reaction, and can thus be reused. Amylase catalyzes the hydrolysis breaking down of starch, glycogen and related polysaccharides into more simple and readily usable forms of sugar. During catalysis, the first step is the substrate s. Restriction enzyme lab report essay example graduateway.
Dip a filter paper disc into the 100 oc catalase and drain the disc on a paper towel. The enzyme and substrate temporarily join to form the enzymesubstrate complex. The substrateenzyme complex then reacts artioli, 2008. Amylase is an enzyme that isfound in our bodies that functions to help the body in the digestion food. Starch will not react with benedicts reagent, so the solution will remain blue. For example, the enzyme amylase will only act on the starch called amylose. After the substrate is bound, the reaction takes place, and then the product is released. The substrate enzyme complex then reacts artioli, 2008. The main enzyme for this lab, peroxidase, is found in many. Amelia houlihan catalase lab report biol 101l studocu.
Effects of temperature, concentration, and varying ph on enzyme activity rate harmony richardson biology. In this lab, you will measure enzyme activity in a solution and measure the effect of five different temperatures. What is the relationship between starch, dextrins and glucose. Effect of enzyme concentration during catalysis, the first step is the substrate s binding to the enzyme e, giving an enzyme substrate complex es. This is one of two mandatory lab reports for the biology 101 lab class. This lab also helps students understand how enzymes break down poisonous substances in the human body and how they help speed up the reaction process using less energy. Overall this lab was relatively successful in determining the effects of enzyme concentration, substrate concentration, temperature. Enzyme activity in addition to closetoed shoes, you are required to wear long pants or skirt ankle length to participate in this lab. Writing a biology lab report a special thanks goes to katie jones, the author of our biology lab report samples. Minimal precipitate was seen to be formed during the experiment on the. Describetherelationshipbetweensubstrateconcentrationandtheinitialreactionrateofanenzyme.
Using a color key, you will be able to determine the speed of the enzyme reaction at these different temperatures. The general procedure for the lab is outlined below, and specific details for each variable follow. The binding provides better chemical conditions to activate the reaction and, in turn, lowers the activation energy artioli, 2008. If we titrate 5 time trials of a reaction of h 2 o 2 and a catalase being stopped with h 2 so 4, then we. The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. The purpose of this lab was to observe the effect of enzyme concentration on the reaction. Enzymecatalysis introductionenzymesareproteinsproducedbylivingcellsthatactascatalysts,whichaffectthe rateofabiochemicalreaction.
Enzyme activity measuring the effect of enzyme concentration. Enzymes lab report inroduction in this lab we explore an enzymes activity and how it can be affected by changes to its environment. The reactant an enzyme acts on is referred to the enzymes substrate. Effect of enzyme concentration during catalysis, the first step is the substrate s binding to the enzyme e, giving an enzymesubstrate complex es. Proxy assay of catalase activity ten 50 ml beakers were prepared, containing graded dilutions of h 2 o 2 from 0.
A stock solution of catalase enzyme solution was obtained 1. In this lab, enzyme kinetics are examined utilizing various experimental techniques, including measurements of absorbance and temperature, to determine the effects on reaction rate dependent on enzyme and substrate concentration, temperature, and substrate specificity, as well as calculate the concentration of enzymes and substrates, v o. In this lab students will investigate a few of the different enzymes from our. The main objective of the experiment is to investigate the enzyme activities and kinetics based on few parameters such that difference in ph, temperature and concentration of substrate. An investigation of catalasehydrogen peroxide enzymatic kinetics. While the two are joined, the substrate is converted to its. It helps accelerate reactions by lowering the activation energy, which is. What is the purpose of having this enzyme in your cells. Draw an energy diagram that shows the energy in a chemical reaction with and without an enzyme.
In this lab students will investigate a few of the different enzymes from our body. Be sure to address the issues highlighted in the lab manual and by your instructor in the. Peroxidase lab report essay sample new york essays. Substrate h 2o 2 hydrogen peroxide catalyst peroxide 3. The amount of h 2 o 2 that decomposed at room temperature 2ml24 hours 7ml5ml2ml activity d. Enzyme catalysis lab report1 university of pennsylvania. Higher or lower ph affects the hydrogen bonding and structure of the enzyme, directly altering the active site where substrate binds, and thus reduces or inhibits the enzymes activity or rate. Enzyme lab report bio181l general biology i studocu. Enzymes biology i laboratory manual lumen learning. The effect of ph on the rate of enzyme catalysis of catalase objectives. The positive control of the experiment was the test tube with the 1.
The purpose of this lab is to investigate the impact of temperature, substrate concentration, enzyme concentration, and the presence of an inhibitor on the effectiveness and rate of an enzyme. This laboratory explores the affects ph has on a reaction rate. More specifically, the objective was to perform an experiment in order to. In this lab, the enzyme catalase was used to break down hydrogen peroxide into less toxic water and oxygen gas. Initially, to understand rates of reaction, an iodine test was completed on solutions containing the enzyme salivary amylase followed by the. The method of determining the michaelis constant, km used in the. Pdf effect of changing substrate concentration on the amount of. The main enzyme for this lab, peroxidase, is found in many different forms, with optimum phs ranging from 4 to 11 depending on the source and optimum temperatures varying from 10 to 70c. Baseline amount of h 2 o 2 present in the solution.